7/22/2023 0 Comments Touchdown pcrUse of degenerate primers and touchdown PCR for construction of cDNA libraries. Comparison of single and touchdown PCR protocols for detecting Pneumocystis jirovecii DNA in paraffin-embedded lung tissue samples. The use of 'touchdown' polymerase chain reaction increases the sensitivity and specificity of t(11 14)(q13 q32) detection in patients with mantle cell lymphoma. Multistep-touchdown vectorette-PCR-a rapid technique for the identification of IVS in genes. Single-tube heminested PCR coupled with 'touchdown' PCR for the analysis of the walleye dermal sarcoma virus env gene. High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR. Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes. Improved nearest-neighbor parameters for predicting DNA duplex stability. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction. Stability of ribonucleic acid double-stranded helices. OligoCalc: an online oligonucleotide properties calculator. Comparison of different melting temperature calculation methods for short DNA sequences. Quantification of mRNA using real-time RT-PCR. Touchdown thermocycling program enables a robust single nucleotide polymorphism typing method based on allele-specific real-time polymerase chain reaction. 'Touchdown' PCR to circumvent spurious priming during gene amplification. Enzymatic amplification of DNA by PCR: standard procedures and optimization. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
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7/22/2023 0 Comments Sonic spinball rom edgeSadly though, whilst we can see the spines of the cases and the names on them, there isn’t any box art to show off on the front, nor is there any description of the game like there was back on the 360 Mega Drive collection.Īnother neat feature though is the ability to change the time of day in the room and the window gives off some cool lighting effects on the various pieces of furniture in the room. Posters of Revenge of Shinobi, Altered Beast and Comix Zone adorn the wall, and on the right-hand side is a bookshelf crammed full of Sega games (with room to spare on the bottom shelf should more be added *fingers crossed*). That isn’t due to the porting or emulation that’s occurred either… that’s down to solid game design back when they were first being created.Įven the main menu screen is unapologetically ‘90s, displaying all the menu options and sub-menus in what would have been a 1990’s kid’s bedroom. Many of the games in this list follow suit, remaining just as playable now as they did back in the early ‘90s. But this was a game that played well back then and it plays just as well now. Granted, looking back now this was due to the technical limitations as the Mega Drive could only produce 61 colours and the sound chip used then is now nothing to write home about. It invoked deeply disturbing thoughts in my head of what would happen if the world got invaded by aliens, what with its bleak presentation and piercing screams of terror. It is at this point I’d like to introduce you to my personal favourite ‘hidden gem’, Alien Storm.Īlien Storm was a game I played regularly round my friend’s house when we younger. But for those who grew up virtually chained to this machine – myself included – there are a lot of hidden gems in the collection. These are all good places to start, and for anyone who has never played these before would get more than their money’s worth just from these alone. These are just the highlights in what is a very strong roster – the Phantasy Star games alone offer over 50 hours of gameplay. This is one collection that does contain plenty of other classics though, such as most other Sonic games – including Sonic Spinball – the Golden Axe series, the three Shining games released on the console, Phantasy Star II-IV, the Streets of Rage trilogy, and arguably most importantly, Toejam and Earl. There are also none of the classic Disney titles that many gamers will have played alongside the games that are featured, but these can be explained away simply by mentioning licensing issues. The Sonic omissions seem inexcusable but in all seriousness, what the hell was Ecco the Dolphin even about? Out Run could also be argued as an omission, but having never seen that released in any other retro collections this isn’t a surprise. It does however have a few notable exceptions such as Sonic 3, Sonic & Knuckles and the Ecco the Dolphin series that are available on the PC collections. SEGA Mega Drive Classics is primarily the same as the collections previously released on Microsoft Windows and through Steam, now rolled up into one overall package. 7/22/2023 0 Comments Happy together 1997An On-Air thread can be made up to 12h prior and 1 week after an episode/movie's release. On-Air Systemĭiscuss recently released shows/movies/comics in dedicated On-Air threads. If it does not directly pertain to BL media, do not post it. Hit pieces generalizing BL and the BL community Music videos with original LGBT characters Solo content of a canonically non-BL character Information/question irrelevant to an actor/creator's BL career Insignificant solo photo(s) of one BL actor This is not an exhaustive list, merely the most frequent offenders: Use the Recs, Reviews, and Chit Chat thread for quick questions, basic recommendation requests, off-topic conversations, and other brief comments. Links related to buying or selling BL media and merch News about upcoming release dates, conventions, etc. Questions related to identification, online access, and translation Reviews, discussion topics, and academic discourse It would be awesome to keep this sub discussion/text heavy, but feel free to post any of the following: This subreddit was created to be a discussion-based spin-off of /r/yaoi. Leave addresses, phone numbers, and other identifiable info to the DM.Max 3 posts per person per 24 hour period.Redact identifying information from content involving non-public figures.No brigading, intentional or otherwise.Please refer to our wiki or sidebar for more information.Use On-Air threads to discuss currently airing shows.Post broad recommendation requests to the pinned Recs, Reviews, and Chit Chat thread.Screenshot or repost the contents instead. Reserve link posts for articles and videos whose contents would not transfer easily to a native Reddit format.Use post flairs and concise titles that describe your post's contents e.g.Our stance on self-promotion can be found here.This is just for reference, though, since the Western BL community is loosey goosey with terminology. All of the above can be discussed here.įor different BL subcategories and specific BL terminology, take a quick peep at our Wiki. Nowadays, all manner of art, comics, anime, games, and dramas from many different countries operate under the BL genre heading. What is Boys' Love?īoys' Love (BL) is by origin a genre of Japanese manga produced mainly by women, for women that features romantic and sexual relationships between men. 7/22/2023 0 Comments A day without me lyricsIt did not appear on the October Tour until November 1981 ( 1981-11), when it was paired with "I Threw a Brick Through a Window", a song from the newly released October album. On the Boy Tour, it did not have a firmly defined set list position, initially appearing in the main set before moving to the encore. "A Day Without Me" was played live during the first half of the 1980s. The image echoes the Joy Division photoshoot on the Epping Walk Bridge in Hulme, Manchester, that featured in an article of New Musical Express in January 1979. The image on the single's cover, photographed by Susan Byrne, is of a footbridge at Booterstown railway station in South Dublin. The B-side is an instrumental song called "Things to Make and Do." The single release was produced by Steve Lillywhite, and was the first time that he recorded with U2. However, this information was erroneous as the band performed the song in Dublin in February 1980, musically and lyrically unaltered from its release format, a few months before Curtis's death. Īt the time of the single's release, an Island Records press officer was notifying enquirers who were corresponding with the record label about the band that the song's lyrics were written about the death of Ian Curtis, the frontman/vocalist of the band Joy Division. The song's lyrics refer to the removal of someone from their habitual social circle, and reflecting on what the world would be like without their presence. It was released as the album's lead single in August 1980, and was their first release to be produced by Steve Lillywhite. " A Day Without Me" is a song by Irish rock band U2, and the eighth track on their debut album Boy. 7/22/2023 0 Comments Binance us stakingIn 2021, nearly 6.5 million ETH was staked in different staking pools whereas, in 2022, the number has vigorously doubled and is presently sits at 13.4 million ETH. Per the data shared by crypto insights firm Arcane Research, the total amount of staked Ethereum has registered a significant surge of 100% in 2022 as compared to the data reported last year. Later on, the exchange evolved to offer some of the most simple and user-friendly mechanisms permitting users to auto-stake tokens in a few simple clicks.Įthereum staking has evolved consistently and has become a popular tool for users to maximize their yields and accrue more profits on their staked assets. The exchange first started offering ETH staking services in June 2022. “ETH plays a critical role in the broader Web3 ecosystem, and as the Ethereum network continues to transition towards The Merge, we are thrilled to now offer ETH staking with some of the highest APY rewards in the industry…We continue to build out a comprehensive staking offering that will provide the most value for our customers.” Shroder later shared Binance’s move to reduce the processing time for ETH staking withdrawals may put pressure on other. The current ETH price is 1826.05, showing a -1.065 decline in the last 24 hours. Ether (ETH) Stakers Flee Crypto Exchanges Binance, Coinbase as Rocket Pool, Frax Finance Gain Deposits Consensus Magazine Learn Bitcoin Calculator Consensus Webinars Indices About Markets Finance. Per the exchange CEO, Brian Shroder, Binance.US is offering one of the highest APYs in the industry to deliver maximum value and benefits to its users. This may lead to more people willing to stake their ETH, which could result in a decrease in the circulating supply of ETH, potentially driving up its price. By launching staking for ETH – one of the world’s most widely held tokens – Binance.US is furthering its commitment to always provide customers with the best products and services.” “While there is a 32 ETH requirement for staking directly through the Ethereum network, users can stake on Binance.US with a competitively low minimum of just 0.001 ETH. Per the official blog post, users can now stake ETH on Binance.US with a low minimum balance of 0.001 ETH. The statement further outlines how Binance.US is narrowing the entry barrier into the staking domain by lowering the minimum ETH requirement by almost 90%. Get Started: /lZfQB6GWGe- Binance.US □□ September 7, 2022 Grow your portfolio while you wait for Ethereum Proof-of-Stake upgrade to launch. Lock in your $ETH for next-level rewards with the highest APY among major #crypto exchanges in the US. 7/22/2023 0 Comments Hill climb racing 2 1.45.2 modHowever, some of the two versions also share several elements. Hill Climb Racing 2 apk moonlander unlocked has several exceptional features that set it apart from the original version. You’ll be able to use these points to unlock more powerful vehicles and enjoy overall gaming features. Ensure that you earn as many points as you can since they’ll be instrumental in enhancing your gameplay. The more races you engage in, the more treasures you’ll garner. You’ll race on many tracks some characterized by Nuclear Plants and Ragnarok. Just like his name suggests, Newton is super determined to defy Newton’s law of gravity! Yes, you’ll join him to climb mountains and conquer highly precarious courses. The racing enthusiast is determined to go to regions where no other racer has ever conquered. In the game, you’ll have a partner called Newton Bill. Also, you can customize it to achieve the best horsepower and torque, thus giving you enough strength to overcome the most challenging terrains. As such, you can be sure that you’ll always have a fresh element in the gameplay to keep you rejuvenated! Interesting Ways to Play Hill Climb Racing 2įortunately straightforward gameplay keeps nyone hooked to Hill Climb Racing 2! You’ll just be required to pick your favourite car from several available options. It is bigger, better, and you’ll undoubtedly have more fun!Īdditionally, the game offers numerous events with different requirements and challenges. This second addition of the popular racing game has incorporated lots of improvements compared to the original version. How resilient are you to hand off-road driving conditions? Download Hill Climb Racing 2 APK unlimited money diamond and fuel for Android and test your skills! Strap in your seatbelt for a racing game that challenges you to race in incredibly hilly and challenging areas. The game features stunning 3D graphics with cartoonish characters that bring a unique charm to its visuals while still providing realistic animations during gameplay moments. There are daily challenges available for extra rewards like special cards used for upgrading character stats even further! So if you’re looking for an enjoyable soccer experience then look no further than Head Ball 2! 3D Graphics : Players can customize their characters with various items such as hats, shirts or shoes which are unlocked through playing matches or purchased from the store using coins earned by winning games. Head Ball 2 Mod Apk offers intense 1v1 real time battles where players must score more goals than their opponent within two minutes of playtime to win points and climb up leaderboards across different leagues around the world. Additionally, there are daily challenges available for extra rewards like special cards used to upgrade character stats even further! So if you’re looking for a fun and competitive experience then look no further than Head Ball 2! Yes, Head Ball 2 Mod Apk is an online multiplayer soccer game where two players compete against each other in real time. Additionally, there are daily challenges available for extra rewards like coins or special cards used for upgrading character stats even further! So if you’re looking for an enjoyable soccer experience then look no further than Head Ball 2! Is it a multiplayer game? The fast-paced gameplay makes it easy to pick up and play while the various customization options allow you to create your own unique character with different looks. Yes, Head Ball 2 Mod Apk is a fun and exciting game that can be enjoyed by players of all ages. Additionally, there are daily challenges available for extra rewards like special cards used for upgrading character stats even further! Play With Fun this game? The objective of the game is to score more goals than your opponent within two minutes and win points that will help you climb up leaderboards across different leagues around the world. Head Ball 2 is a fast-paced soccer game where two players compete against each other in real time. Head Ball 2 also offers daily challenges where players have an opportunity to earn rewards like coins or special cards used for upgrading character stats even further! Gameplay Overview Players compete against each other in 1v1 real time battles where they must score more goals than their opponent within two minutes of playtime to win points and climb up leaderboards across different leagues around the world. It features 3D graphics with cartoonish characters that can be customized to the player’s liking using various items such as hats, shirts or shoes which are unlocked through playing matches or purchased from the store. The game was released in 2018 for iOS, Android, Microsoft Windows and macOS platforms. Head Ball 2 Mod Apk is a free-to-play online multiplayer soccer game developed and published by Masomo Gaming. Head Ball 2 Mod Apk 1.500 (Unlimited Coins) APP INFO: Head Ball 2 Mod Apk Q Are there any ads while playing this game?.Q Can I customize my characters with different looks ?.Head Ball 2 Mod Apk 1.500 (Unlimited Coins). 7/22/2023 0 Comments Free shutter counter eos 5d markiiSince ML is third party software there is some risk in using it. The original version of magic Lantern was developed for the EOS 5D Mark II and is perhaps the most stable version of ML there is. To use Magic Lantern to access the shutter count you must have one of the cameras listed above for which there is also a version of Magic Lantern available. You can also access the internal shutter count on certain EOS cameras using Magic Lantern. However, there are some security concerns floating around the internet about that program's developer so I chose to stop using it. Prior to buying ShutterCount I was using a free app called EOSInfo. In fact, the older version compatible with Windows is no longer offered for sale by Dire Studio.ĭire Studio also now offers an add-on Live View Pack to their Shutter Count app that allows one to see Live View stills and Live View movie actuations of the shutters in selected cameras. They once had a statement to this effect included in one of the FAQs, but it seems to no longer be there at their site.Īs of 22 February, 2018 there has still been no update to the Windows version of Shutter Count. Unfortunately, it doesn't look like Dire Studios is interested in ever updating the Windows version again, due to the high cost of Microsoft licensing. The Windows version will support these cameras later on.įor a list of all supported cameras, please see the Compatibility List at the ShutterCount Tech Specs page. Update to ShutterCount 3.0 or later on your Mac or iOS device. Q13 - The shutter count is not displayed for new cameras (5D IV, 5DS/R, 1D X II, etc.) From the updated FAQ page as of July 2, 2017: Updateĭire Studio has released ShutterCount version 3 (currently for Mac or iOS only) that can now read the shutter counts for the cameras excluded above, as well as some other more recent models. I've been using it for about a year and a half and am very happy with it. As of Decemthe Windows version is $3.99 USD and all other versions are $2.99 USD. Although it is not free, the cost is very modest. The aforementioned Dire Studio offers an app called ShutterCount that works with compatible Canon EOS cameras listed above. Only the Canon service centers can extract that info from EOS cameras. Live View actuations are not reported via the remote control interface. Your EOS Rebel T3i/600D falls into the group that allows the remote control application to access the camera's shutter count for still images taken with the camera. For more detailed information about specific models, please see this page at dire studio's website. None of the models released since early 2015 with DiG!C 6/6+ or later processors allow the shutter count to be accessed without the proprietary tools used by Canon service centers. The EOS 7D Mark II is the only DiG!C 6/6+ camera that allows shutter count access via the remote controlled interface. This includes all DiG!C III, DiG!C 4, and DiG!C 5/5+ cameras. The remote control interface may also be accessed via WiFi connection for cameras so equipped with WiFi capability. The shutter count from EOS cameras with DiG!C III and later processors up to cameras released by the end of 2014 make the shutter count available through the remote controlled interface that may be accessed on the camera via a USB connection. So online solutions where the user uploads a photo from the camera in question or other methods dependent upon a "shutter count" field in the EXIF info do not work with EOS camera. The shutter count is not included in the EXIF information of images made using EOS cameras such as is the case with some cameras from some other manufacturers. 7/22/2023 0 Comments Sirds imagesPress clips : OPINION - How to cultivate agri-startups by Dr M Srikanth, Associate Professor and Director (Finance), DDU-GKY, National Institute of Rural Development and Panchayati Raj, Hyderabad, and Dr Khanindra Ch Das, Assistant Professor, Birla Institute of Management Technology, New Delhi. Press release : National Institute of Rural Development and Panchayati Raj (NIRDPR) and Indian Pest Control Association (IPCA) signed a memorandum of understanding (MoU) on Wednesday, 11 th May on collaborative initiatives in the field of Pest Management Sector, particularly to standardize skill development for various job roles in the sector. Press release : Regional Capacity Building Training Program "Inspiring the Minds for Disaster Risk Reduction" begins at NIRDPR on May 17. Press clips : Hon'ble Union Minister for Rural Development Shri Giriraj Singh visit to NIRDPR Press release : Hon'ble Union Minister for Rural Development & Panchayati Raj inaugurates PMAY-G Model House at NIRDPR. Press release : National Institute of Rural Development and Panchayati Raj is facilitating six days training cum study tour of 21 Officers of Bihar Administrative Service to Telangana State from 27 th June to 2 nd July 2022. Press note : NIRDPR has been awarded as best RSA for Andhra Pradesh on 41 st Foundation Day of NABARD. Chandra Shekhar Kumar, IAS, Additional Secretary, Ministry of Panchayati Raj (MoPR) Government of India. Press release : Hyderabad, 12 th July 2022 - A national level Training of Trainers (ToT) Program for Localization of Sustainable Development Goals (LSDG) has been inaugurated at NIRDPR, Hyderabad by Dr. Press release : NIRDPR Organized National Consultative Workshop on Capacity Building of Stakeholder for Educational Development in Rural Areas.Ĭabinet approves signing of MoU between National Institute of Rural Development and Panchayati Raj (NIRD&PR) and University of Reading (UoR), United Kingdom (UK). Press clips : Hindi day celebrations at NIRDPR. Press clips : NIRDPR inks pact with National Health Systems Resource Centre to promote health care in rural areas. Sounds like a separate packs hosted with Matplotlib might be the best way to go.Press clips : NIRDPR releases study on Rural Transformation stories in PM's Mann Ki Baat. Any resampling or removal of dots (they can change in size, but not presence) will likely corrupt the visual output. Your comment about the image routine Re-Sampling could be a problem as the output image size needs to be known and the "random" dots are selected to appropriately encode the height data. Ultimately, you pass this a 2D array with "height data" encoded at each point and it creates a 2D image of random dots for display which encodes that height data. This was the intent for this project doesn't depend on TensorFlow, but just happen to be the package I was working with at the time. It is useful for viewing "Water-Fall" type of plots. I am looking for a way to make this available to the scientific community and let them know it is available. The size isn't that big for the code, maybe a 2K or so. I could use some pointers to get started. Thanks for your A separate package might work. I really have no idea where to start to get this into MatPlotLib and would like to work with someone to help integrate the code if desired. If there is interest from the community, I am more than willing to provide the code to help generate this output format. It dawned on me that this tool would be much more available to the scientific community it it were included in more generic visualization tools such as MatPlotLib. You can see an example of what I mean on my GitHub page: Data is encoded to show data in the Z direction as depth. It allows for scientific data display of a waterfall type of plot with no hidden lines due to perspective. SIRDS is a means to present 3D data in a 2D image. Single Image Random Dot Stereogram (SIRDS) I have coded a Single Image Random Dot Stereogram C++ function for TensorFlow to produce 3D SIRDS images on a 2D plan. 7/21/2023 0 Comments I am fish xbox game passSwim, roll, glide, chomp, flip flop, inflate, fly and bite your way through enthralling challenges. Flying Fish – a little aloof at times but a real softy at heart, with the ability to glide through the air! These plucky heroes will leave no bowl unturned, putting their heart and shoal into the mission to re-unite! Piranha - wild, chaotic, loud, unpredictable, and loves to bite - obviously. Meet our heroes! Goldfish - cheerful, brave, and adventurous, a natural born swimmer! Pufferfish – a little slow but kind-hearted who can also puff up into a ball and roll across land. Over the course of the game you join them as they swim, fly, roll and chomp their way to the open ocean from the far-flung corners of Barnardshire (the smallest county in England) in their bid for freedom and to re-unite once again. I am Fish is a charming, physics-based adventure starring four intrepid fish friends, forcibly separated from their home in a pet shop fish tank. Click to share on Email (Opens in new window). Click to share on Facebook (Opens in new window).Click to share on Twitter (Opens in new window).Whilst I Am Fish may look like a piece of hake to play, actshoally the controls are deliberately designed to be challenging and with an additional layer of added Bossa Style controls, it won’t be like shooting fish in a barrel. Cheers! Navigating in each make-shift aquatic vehicle poses its own unique challenge, but don’t worry, the relative freedom of open water is never too far away with inviting fountains, swimming pools and err, sewers full of hazardous materials to splash around in, before reaching the final goal – the shimmering, open ocean. Our four fin-tastic friends will be hitching rides in all manner of ad hoc water carriers including jars, mop buckets on wheels and the occasional pint glass. We won’t dwell on grizzly fish death, but should your fish fall too far in whatever make-shift fishbowl it finds itself in, run out of air or generally flounder you will be popped back to the most recent checkpoint to refine your approach. With its idyllic coastline and quaint villages Barnardshire might seem like a perfect slice of quintessential tranquility, but to our aquatic adventurers it’s alive with the very real threats presented by crossing roads, traversing rooftops, dodging deep fat fryers, avoiding wildlife including the cantankerous locals, not to mention fragile fishbowls and the very real problem that fish can’t breathe out of water. A simple, intuitive control scheme leaves no excuses should your fish perish leaving you feeling very gill-ty. |