7/22/2023 0 Comments Touchdown pcrUse of degenerate primers and touchdown PCR for construction of cDNA libraries. Comparison of single and touchdown PCR protocols for detecting Pneumocystis jirovecii DNA in paraffin-embedded lung tissue samples. The use of 'touchdown' polymerase chain reaction increases the sensitivity and specificity of t(11 14)(q13 q32) detection in patients with mantle cell lymphoma. Multistep-touchdown vectorette-PCR-a rapid technique for the identification of IVS in genes. Single-tube heminested PCR coupled with 'touchdown' PCR for the analysis of the walleye dermal sarcoma virus env gene. High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR. Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes. Improved nearest-neighbor parameters for predicting DNA duplex stability. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction. Stability of ribonucleic acid double-stranded helices. OligoCalc: an online oligonucleotide properties calculator. Comparison of different melting temperature calculation methods for short DNA sequences. Quantification of mRNA using real-time RT-PCR. Touchdown thermocycling program enables a robust single nucleotide polymorphism typing method based on allele-specific real-time polymerase chain reaction. 'Touchdown' PCR to circumvent spurious priming during gene amplification. Enzymatic amplification of DNA by PCR: standard procedures and optimization. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
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